Plasma membrane-localized plant immune receptor targets H⁺ -ATPase for membrane depolarization to regulate cell death.

ABSTRACT: The hypersensitive response (HR) is a robust immune response mediated by nucleotide-binding, leucine-rich repeat receptors (NLRs). However, the early molecular event that links activated NLRs to cell death is unclear. Here, we demonstrate that NLRs target plasma membrane H⁺ -ATPases (PMAs) that generate electrochemical potential, an essential component of living cells, across the plasma membrane. CC^A 309, an autoactive N-terminal domain of a coiled-coil NLR (CNL) in pepper, is associated with PMAs. Silencing or overexpression of PMAs reversibly affects cell death induced by CC^A 309 in Nicotiana benthamiana. CC^A 309-induced extracellular alkalization causes plasma membrane depolarization, followed by cell death. Coimmunoprecipitation analyses sugg

SUBMITTER: Lee HY 

PROVIDER: S-EPMC9298278 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature