Development and validation of a rapid LC-MS/MS method for the detection of 182 novel psychoactive substances in whole blood.

ABSTRACT:

Introduction

The analysis of novel psychoactive substances (NPS) represents a challenge in forensic toxicology, due to the high number of compounds characterized by different structures and physicochemical properties both among different subclasses and within a single subclass of NPS. The aim of the present work is the development and validation of a targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection of NPS in whole blood.

Materials and methods

A protein-precipitation based LC-MS/MS method for the detection of more than 180 NPS was developed and validated by assessing the following parameters: selectivity, linearity, accuracy, precision, limit of detection (LOD) and of quantification (LOQ) recovery, and matrix effect. Then, the method

SUBMITTER: Giorgetti A 

PROVIDER: S-EPMC9298299 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature