Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts.

ABSTRACT:

Background

There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.

Methods

Cardioplegia-arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.

Results

Fo

SUBMITTER: van Suylen V 

PROVIDER: S-EPMC9298357 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature