Analysis of the Genomic Sequence of ABO Allele Using Next-Generation Sequencing Method.

ABSTRACT:

Background

Although many molecular diagnostic methods have been used for ABO genotyping, there are few reports on the full-length genomic sequence analysis of the ABO gene. Recently, next-generation sequencing (NGS) has been shown to provide fast and high-throughput results and is widely used in the clinical laboratory. Here, we established an NGS method for analyzing the sequence of the start codon to the stop codon in the ABO gene.

Study design and methods

Two pairs of primers covering the partial 5'-untranslated region (UTR) to 3'-UTR of the ABO gene were designed. The sequences covering from the start codon to the stop codon of the ABO gene were amplified using these primers, and an NGS method based on the overlap amplicon was developed

SUBMITTER: He Y 

PROVIDER: S-EPMC9298404 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature