Lipidomic profiling of exosomes from colorectal cancer cells and patients reveals potential biomarkers.

ABSTRACT: Strong evidence suggests that differences in the molecular composition of lipids in exosomes depend on the cell type and has an influence on cancer initiation and progression. Here, we analyzed by liquid chromatography-mass spectrometry (LC-MS) the lipidomic signature of exosomes derived from the human cell lines normal colon mucosa (NCM460D), and colorectal cancer (CRC) nonmetastatic (HCT116) and metastatic (SW620), and exosomes isolated from the plasma of nonmetastatic and metastatic CRC patients and healthy donors. Analysis of this exhaustive lipid study highlighted changes in some molecular species that were found in the cell lines and confirmed in the patients. For example, exosomes from primary cancer patients and nonmetastatic cells compared with healthy donors and control cells dis

SUBMITTER: Elmallah MIY 

PROVIDER: S-EPMC9298677 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature