A Modular System for the Rapid Comparison of Different Membrane Anchors for Surface Display on Escherichia coli.

ABSTRACT: Comparison of different membrane anchor motifs for the surface display of a protein of interest (passenger) is crucial for achieving the best possible performance. However, generating genetic fusions of the passenger to various membrane anchors is time-consuming. We herein employ a recently developed modular display system, in which the membrane anchor and the passenger are expressed separately and assembled in situ via SpyCatcher and SpyTag interaction, to readily combine a model passenger cytochrome P450 BM3 (BM3) with four different membrane anchors (Lpp-OmpA, PgsA, INP and AIDA-I). This approach has the significant advantage that passengers and membrane anchors can be freely combined in a modular fashion without the need to generate direct genetic fusion constructs in each case. We dem

SUBMITTER: Gallus S 

PROVIDER: S-EPMC9298812 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature