Binding Methylarginines and Methyllysines as Free Amino Acids: A Comparative Study of Multiple Host Classes.

ABSTRACT: Methylated free amino acids are an important class of targets for host-guest chemistry that have recognition properties distinct from those of methylated peptides and proteins. We present comparative binding studies for three different host classes that are each studied with multiple methylated arginines and lysines to determine fundamental structure-function relationships. The hosts studied are all anionic and include three calixarenes, two acyclic cucurbiturils, and two other cleft-like hosts, a clip and a tweezer. We determined the binding association constants for a panel of methylated amino acids using indicator displacement assays. The acyclic cucurbiturils display stronger binding to the methylated amino acids, and some unique patterns of selectivity. The two other cleft-like hosts

SUBMITTER: Warmerdam Z 

PROVIDER: S-EPMC9299052 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature