Scaling-up a fragment-based protein-protein interaction method using a human reference interaction set.

ABSTRACT: Protein-protein interactions (PPIs) are essential in understanding numerous aspects of protein function. Here, we significantly scaled and modified analyses of the recently developed all-vs-all sequencing (AVA-Seq) approach using a gold-standard human protein interaction set (hsPRS-v2) containing 98 proteins. Binary interaction analyses recovered 20 of 47 (43%) binary PPIs from this positive reference set (PRS), comparing favorably with other methods. However, the increase of 20× in the interaction search space for AVA-Seq analysis in this manuscript resulted in numerous changes to the method required for future use in genome-wide interaction studies. We show that standard sequencing analysis methods must be modified to consider the possible recovery of thousands of positives among million

SUBMITTER: Schaefer-Ramadan S 

PROVIDER: S-EPMC9299658 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature