Proteasome inhibition alters mitotic progression through the upregulation of centromeric α-Satellite RNAs.

ABSTRACT: Cell cycle progression requires control of the abundance of several proteins and RNAs over space and time to properly transit from one phase to the next and to ensure faithful genomic inheritance in daughter cells. The proteasome, the main protein degradation system of the cell, facilitates the establishment of a proteome specific to each phase of the cell cycle. Its activity also strongly influences transcription. Here, we detected the upregulation of repetitive RNAs upon proteasome inhibition in human cancer cells using RNA-seq. The effect of proteasome inhibition on centromeres was remarkable, especially on α-Satellite RNAs. We showed that α-Satellite RNAs fluctuate along the cell cycle and interact with members of the cohesin ring, suggesting that these transcripts may take part in the

SUBMITTER: Caceres-Gutierrez RE 

PROVIDER: S-EPMC9299679 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature