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Engineering ergothioneine production in Yarrowia lipolytica.

ABSTRACT: Ergothioneine is a naturally occurring antioxidant that has shown potential in ameliorating neurodegenerative and cardiovascular diseases. In this study, we investigated the potential of the Crabtree-negative, oleaginous yeast Yarrowia lipolytica as an alternative host for ergothioneine production. We expressed the biosynthetic enzymes EGT1 from Neurospora crassa and EGT2 from Claviceps purpurea to obtain 158 mg·L^-1 of ergothioneine in small-scale cultivation, with an additional copy of each gene improving the titer to 205 mg·L^-1 . The effect of phosphate limitation on ergothioneine production was studied, and finally, a phosphate-limited fed-batch fermentation in 1 L bioreactors yielded 1.63 ± 0.04 g·L^-1 ergothioneine in 220 h, corresponding to an overall volumetric productivity of 7.41 mg·L^-1 ·h^-1 , showing that Y. lipolytica is a promising host for ergothioneine production.

SUBMITTER: van der Hoek SA

PROVIDER: S-EPMC9299812 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Engineering ergothioneine production in Yarrowia lipolytica.

van der Hoek Steven A SA Rusnák Matej M Jacobsen Irene Hjorth IH Martínez José L JL Kell Douglas B DB Borodina Irina I

FEBS letters 20211205 10

Ergothioneine is a naturally occurring antioxidant that has shown potential in ameliorating neurodegenerative and cardiovascular diseases. In this study, we investigated the potential of the Crabtree-negative, oleaginous yeast Yarrowia lipolytica as an alternative host for ergothioneine production. We expressed the biosynthetic enzymes EGT1 from Neurospora crassa and EGT2 from Claviceps purpurea to obtain 158 mg·L-1 of ergothioneine in small-scale cultivation, with an additional copy ...[more]

PMID: 34817066

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