Engineering ergothioneine production in Yarrowia lipolytica.

ABSTRACT: Ergothioneine is a naturally occurring antioxidant that has shown potential in ameliorating neurodegenerative and cardiovascular diseases. In this study, we investigated the potential of the Crabtree-negative, oleaginous yeast Yarrowia lipolytica as an alternative host for ergothioneine production. We expressed the biosynthetic enzymes EGT1 from Neurospora crassa and EGT2 from Claviceps purpurea to obtain 158 mg·L^-1 of ergothioneine in small-scale cultivation, with an additional copy of each gene improving the titer to 205 mg·L^-1 . The effect of phosphate limitation on ergothioneine production was studied, and finally, a phosphate-limited fed-batch fermentation in 1 L bioreactors yielded 1.63 ± 0.04 g·L^-1 ergothioneine in 220 h, corresponding to an overall

SUBMITTER: van der Hoek SA 

PROVIDER: S-EPMC9299812 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature