Regioselective C4 and C6 Double Oxidation of Cellulose by Lytic Polysaccharide Monooxygenases.

ABSTRACT: Lytic polysaccharide monooxygenases (LPMOs) play a key role in enzymatic degradation of hard-to-convert polysaccharides, such as chitin and cellulose. It is widely accepted that LPMOs catalyze a single regioselective oxidation of the C1 or C4 carbon of a glycosidic linkage, after which the destabilized linkage breaks. Here, a series of novel C4/C6 double oxidized cello-oligosaccharides was discovered. Products were characterized, aided by sodium borodeuteride reduction and hydrophilic interaction chromatography coupled to mass spectrometric analysis. The C4/C6 double oxidized products were generated by C4 and C1/C4 oxidizing LPMOs, but not by C1 oxidizing ones. By performing incubation and reduction in H₂ ¹⁸ O, it was confirmed that the C6 gem-diol structure resulted

SUBMITTER: Sun P 

PROVIDER: S-EPMC9299857 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature