Electrochemiluminescence resonance energy transfer biosensing platform between g-C₃N₄ nanosheet and Ru-SiO₂@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene.

ABSTRACT: Rational detection of syndrome coronavirus 2 (SARS-CoV-2) is crucial to prevention, control, and treatment of disease. Herein, a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) between g-C₃N₄ nanosheets and Ru-SiO₂@folic acid (FA) nanomaterials was designed to realize ultrasensitive detection of SARS-CoV-2 virus (RdRp gene). Firstly, the unique g-C₃N₄ nanosheets displayed very intense and stable ECL at 460 nm, then the triple helix DNA was stably and vertically bound to g-C₃N₄ on electrode by high binding affinity between ssDNA and g-C₃N₄. Meanwhile, trace amounts of target genes were converted to a large number of output by three-

SUBMITTER: Yin T 

PROVIDER: S-EPMC9299981 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature