Freqpcr: Estimation of population allele frequency using qPCR ΔΔCq measures from bulk samples.

ABSTRACT: PCR techniques, both quantitative (qPCR) and nonquantitative, have been used to estimate the frequency of a specific allele in a population. However, the labour required to sample numerous individuals and subsequently handle each sample renders the quantification of rare mutations (e.g., pesticide resistance gene mutations at the early stages of resistance development) challenging. Meanwhile, pooling DNA from multiple individuals as a "bulk sample" combined with qPCR may reduce handling costs. The qPCR output for a bulk sample, however, contains uncertainty owing to variations in DNA yields from each individual, in addition to measurement errors. In this study, we have developed a statistical model to estimate the frequency of the specific allele and its confidence interval when the sample

SUBMITTER: Sudo M 

PROVIDER: S-EPMC9300209 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature