Comparison of constitutive promoter activities and development of maize ubiquitin promoter- and Gateway-based binary vectors for rice.

ABSTRACT: In transgenic experiments, we often face fundamental requirements such as overexpressing a certain gene, developing organelle markers, testing promoter activities, introducing large genomic fragments, and combinations of them. To fulfill these multiple requirements in rice, we developed simple binary vectors with or without maize ubiquitin (UBQ) promoter, Gateway cassette and fluorescent proteins. First, we compared stabilities of cauliflower mosaic virus 35S and maize UBQ promoters for constitutive gene expression in transgenic rice. We show that the 35S promoter was frequently silenced after shoot regeneration, whereas maize UBQ promoter achieved stable expression in various young tissues. Binary vectors with Gateway cassettes under the control o

SUBMITTER: Tsuda K 

PROVIDER: S-EPMC9300420 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature