Introduction of a long synthetic repetitive DNA sequence into cultured tobacco cells.

ABSTRACT: Genome information has been accumulated for many species, and these genes and regulatory sequences are expected to be applied in plants by enhancing or creating new metabolic pathways. We hypothesized that manipulating a long array of repetitive sequences using tethered chromatin modulators would be effective for robust regulation of gene expression in close proximity to the arrays. This approach is based on a human artificial chromosome made of long synthetic repetitive DNA sequences in which we manipulated the chromatin by tethering the modifiers. However, a method for introducing long repetitive DNA sequences into plants has not yet been established. Therefore, we constructed a bacterial artificial chromosome-based binary vector in Escherichia coli cells to generate a construct i

SUBMITTER: Ohzeki J 

PROVIDER: S-EPMC9300429 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature