Cell-type specific changes in PKC-delta neurons of the central amygdala during alcohol withdrawal.

ABSTRACT: The central amygdala (CeA) contains a diverse population of cells, including multiple subtypes of GABAergic neurons, along with glia and epithelial cells. Specific CeA cell types have been shown to affect alcohol consumption in animal models of dependence and may be involved in negative affect during alcohol withdrawal. We used single-nuclei RNA sequencing to determine cell-type specificity of differential gene expression in the CeA induced by alcohol withdrawal. Cells within the CeA were classified using unbiased clustering analyses and identified based on the expression of known marker genes. Differential gene expression analysis was performed on each identified CeA cell-type. It revealed differential gene expression in astrocytes and GABAergic neurons associated with alcohol withdrawal.

SUBMITTER: Dilly GA 

PROVIDER: S-EPMC9300707 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature