Transcriptome Analysis of Tumor-Infiltrating Lymphocytes Identifies NK Cell Gene Signatures Associated With Lymphocyte Infiltration and Survival in Soft Tissue Sarcomas.

ABSTRACT:

Purpose

Clinical successes using current T-cell based immunotherapies have been limited in soft tissue sarcomas (STS), while pre-clinical studies have shown evidence of natural killer (NK) cell activity. Since tumor immune infiltration, especially tumor-infiltrating lymphocytes, is associated with improved survival in most solid tumors, we sought to evaluate the gene expression profile of tumor and blood NK and T cells, as well as tumor cells, with the goal of identifying potential novel immune targets in STS.

Experimental design

Using fluorescence-activated cell sorting, we isolated blood and tumor-infiltrating CD3^-CD56⁺ NK and CD3⁺ T cells and CD45^- viable tumor cells from STS patients undergoing surgery. We then evaluated differ

SUBMITTER: Judge SJ 

PROVIDER: S-EPMC9300876 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature