AP1G2 Affects Mitotic Cycles of Female and Male Gametophytes in Arabidopsis.

ABSTRACT: During sexual reproduction in flowering plants, haploid spores are formed from meiosis of spore mother cells. The spores then undergo mitosis, develop into female and male gametophytes, and give rise to seeds after fertilization. We identified a female sterile mutant ap1g2-4 from EMS mutagenesis, and analyses of two T-DNA insertion mutants, ap1g2-1 ^+/- and ap1g2-3 ^-/-, and detected a partial female and male sterility. The ap1g2 mutant gametophyte development was arrested at one nuclear stage. A complementation test using a genomic sequence of AP1G2 with its native promoter restored the function in the three ap1g2 mutant lines. Transcriptome profiling of ap1g2 ovules revealed that four genes encoding clathrin assembly pr

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC9301471 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature