A sensitive and adaptable method to measure platelet-fibrin clot contraction kinetics.

ABSTRACT:

Background

Platelet-fibrin clot contraction is critical for wound closure and maintenance of vessel patency, yet a molecular understanding of the process has lagged because of a lack of flexible quantitative assay systems capable of assaying multiple samples simultaneously.

Objectives

We devised a sensitive and inexpensive method to assess clot contraction kinetics under multiple conditions.

Methods

Clot contraction was measured using time-lapse digital photography, automated image processing with customized software, and detailed kinetic analysis using available commercial programs.

Results

Our system was responsive to alterations in platelet counts and calcium, fibrinogen, and thrombin concentrations, and our analysis detected and defined three phases of plat

SUBMITTER: Prakhya KS 

PROVIDER: S-EPMC9301529 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature