Spatial genomics enables multi-modal study of clonal heterogeneity in tissues.

ABSTRACT: The state and behaviour of a cell can be influenced by both genetic and environmental factors. In particular, tumour progression is determined by underlying genetic aberrations^1-4 as well as the makeup of the tumour microenvironment^5,6. Quantifying the contributions of these factors requires new technologies that can accurately measure the spatial location of genomic sequence together with phenotypic readouts. Here we developed slide-DNA-seq, a method for capturing spatially resolved DNA sequences from intact tissue sections. We demonstrate that this method accurately preserves local tumour architecture and enables the de novo discovery of distinct tumour clones and their copy number alterations. We then apply slide-DNA-seq to a mouse model of metastasis and a primary

SUBMITTER: Zhao T 

PROVIDER: S-EPMC9301586 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature