Tomato Oxalyl-CoA Synthetase Degrades Oxalate and Affects Fruit Quality.

ABSTRACT: Acyl activating enzyme 3 (AAE3) encodes oxalyl-CoA synthetase involved in oxalate degradation. In this study, we investigated the role of AAE3 (SlAAE3) in the fruit quality of tomato (Solanum lycopersicum). The purified recombinant SlAAE3 protein from Escherichia coli exhibited a high activity toward oxalate, with a K _m of 223.8 ± 20.03 μm and V _max of 7.908 ± 0.606 μmol mg^-1 protein min^-1. Transient expression of SlAAE3-green fluorescent protein (GFP) fusion proteins suggests that SlAAE3 is a soluble protein without specific subcellular localization. The expression of SlAAE3 is both tissue- and development-dependent, and increased during fruit ripping. The Slaae3 knockout mutants had improved fruit quality as e

SUBMITTER: Li P 

PROVIDER: S-EPMC9301600 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature