Additive Manufacturing of Viscoelastic Polyacrylamide Substrates for Mechanosensing Studies.

ABSTRACT: Polymerized polyacrylamide (PAA) substrates are linearly elastic hydrogels that are widely used in mechanosensing studies due to their biocompatibility, wide range of functionalization capability, and tunable mechanical properties. However, such cellular response on purely elastic substrates, which do not mimic the viscoelastic living tissues, may not be physiologically relevant. Because the cellular response on 2D viscoelastic PAA substrates remains largely unknown, we used stereolithography (SLA)-based additive manufacturing technique to create viscoelastic PAA substrates with tunable mechanical properties that allow us to identify physiologically relevant cellular behaviors. Three PAA substrates of different complex moduli were fabricated by SLA. By embedding fluorescent markers during

SUBMITTER: Protick FK 

PROVIDER: S-EPMC9301700 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature