Regulation of gingival fibroblast phenotype by periodontal ligament cells in vitro.

ABSTRACT:

Objectives

Stem cell transplantation has shown modest effects on periodontal tissue regeneration, and it is still unclear how regenerative effects utilizing this modality are mediated. A greater understanding of the basic interactions between implanted and host cells is needed to improve future strategies. The aims of this study were to investigate the effects of periodontal ligament (PDL) cells on expression of periodontal markers and alkaline phosphatase (ALP) activity of gingival fibroblasts (GF).

Materials and methods

Primary human PDL cells were co-cultured with primary GF cultures either by direct co-culture with subsequent FACS sorting or indirect co-culture using transwell cultures and PDL cell conditioned medium. Expression of periodontal markers, asporin, nestin, a

SUBMITTER: Garna DF 

PROVIDER: S-EPMC9302626 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature