When barcoding fails: Genome chimerization (admixing) and reticulation obscure phylogenetic and taxonomic relationships.

ABSTRACT: DNA barcoding is based on the premise that the barcode sequences can distinguish individuals (strains) of different species because their sequence variation between species exceeds that within species. The primary barcodes used in fungal and yeast taxonomy are the ITS segments and the LSU (large subunit) D1/D2 domain of the homogenized multicopy rDNA repeats. The secondary barcodes are conserved segments of protein-encoding genes, which usually have single copies in haploid genomes. This study shows that the analysis of barcode sequences fails to reconstruct accurate species trees and differentiate species when the organisms have chimeric genomes composed of admixed mosaics of different origins. It is shown that the type strains of 10 species of the pulcherrima clade of the ascomycetous ye

SUBMITTER: Sipiczki M 

PROVIDER: S-EPMC9303175 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature