Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles.

ABSTRACT:

Background

Platelets contain a high amount of potentially active A subunit dimer of coagulation factor XIII (cellular FXIII; cFXIII). It is of cytoplasmic localization, not secreted, but becomes translocated to the surface of platelets activated by convulxin and thrombin (CVX+Thr).

Objective

To explore the difference in cFXIII translocation between receptor mediated and non-receptor mediated platelet activation and if translocation can also be detected on platelet-derived microparticles. Our aim was also to shed some light on the mechanism of cFXIII translocation.

Methods

Gel-filtered platelets were activated by CVX+Thr or Ca²⁺ -ionophore (calcimycin). The translocation of cFXIII and phosphatidylserine (PS) to the surface of activated platelets and platelet

SUBMITTER: Somodi L 

PROVIDER: S-EPMC9303193 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature