How low is really low? Comparison of two C-peptide assays to establish residual C-peptide production in type 1 diabetes.

ABSTRACT:

Introduction

C-peptide is an important marker to assess residual insulin production in individuals with type 1 diabetes (T1D). The accuracy and detection limits of C-peptide assays are important to detect C-peptide microsecretion and to reliably observe changes over time in these people. We compared and verified two commercially available assays able to measure C-peptide in the picomolar range.

Methods

The ultrasensitive Mercodia enzyme-linked immunosorbent C-peptide assay (ELISA) was compared with the Beckman immunoradiometric assay (IRMA) for C-peptide, assessing reproducibility (coefficient of variation [CV]), limit of blank (LoB), limit of detection (LoD) and limit of quantitation (LoQ).

Results

For both assays within-run and between-run variation were high at the

SUBMITTER: de Leur K 

PROVIDER: S-EPMC9303196 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature