Dual DNA staining enables isolation of multiple sub-types of post-replicative mouse male germ cells.

ABSTRACT: During spermatogenesis, mammalian male germ cells undergo multiple developmental processes, including meiosis and post-meiotic differentiation (spermiogenesis). To understand the transitions between different cellular states it is essential to isolate pure populations of cells at different stages of development. Previous approaches enabled the isolation of cells from different stages of meiotic prophase I, but techniques to sub-fractionate unfixed, post-meiotic spermatids have been lacking. Here we report the development of a protocol enabling simultaneous isolation of cells at different stages of meiotic prophase and post-meiotic differentiation from testes of adult mice. This approach builds on existing fluorescence activated cell sorting protocols designed to purify cells in different s

SUBMITTER: Gill ME 

PROVIDER: S-EPMC9303198 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature