Characterizing locus specific chromatin structure and dynamics with correlative conventional and super-resolution imaging in living cells.

ABSTRACT: The dynamic rearrangement of chromatin is critical for gene regulation, but mapping both the spatial organization of chromatin and its dynamics remains a challenge. Many structural conformations are too small to be resolved via conventional fluorescence microscopy and the long acquisition time of super-resolution photoactivated localization microscopy (PALM) precludes the structural characterization of chromatin below the optical diffraction limit in living cells due to chromatin motion. Here we develop a correlative conventional fluorescence and PALM imaging approach to quantitatively map time-averaged chromatin structure and dynamics below the optical diffraction limit in living cells. By assigning localizations to a locus as it moves, we reliably discriminate between bound and unbound d

SUBMITTER: Mehra D 

PROVIDER: S-EPMC9303368 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature