Altered protein expression of Streptococcus oralis cultured at low pH revealed by two-dimensional gel electrophoresis.

ABSTRACT: Streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. To identify proteins that are differentially expressed by S. oralis growing under conditions of low pH, soluble cellular proteins extracted from bacteria grown in batch culture at pH 5.2 or 7.0 were analyzed by two-dimensional (2-D) gel electrophoresis. Thirty-nine proteins had altered expression at low pH; these were excised, digested with trypsin using an in-gel protocol, and further analyzed by peptide mass fingerprinting using matrix-assisted laser desorption ionization mass spectrometry. The resulting fingerprints were compared with the genomic database for Streptococcus p

SUBMITTER: Wilkins JC 

PROVIDER: S-EPMC93034 | biostudies-literature | 2001 Aug

REPOSITORIES: biostudies-literature