Multiparameter platelet function analysis of bleeding patients with a prolonged platelet function analyser closure time.

ABSTRACT: Patients referred for evaluation of bleeding symptoms occasionally have a prolonged platelet function analyser (PFA) closure time, without evidence for von Willebrand disease or impaired platelet aggregation. The aim of this study was to establish a shear-dependent platelet function defect in these patients. Patients were included based on high bleeding score and prior PFA prolongation. Common tests of von Willebrand factor (VWF) and platelet function and exome sequencing were performed. Microfluidic analysis of shear-dependent collagen-induced whole-blood thrombus formation was performed. In 14 PFA-only patients, compared to healthy volunteers, microfluidic tests showed significantly lower platelet adhesion and thrombus formation parameters. This was accompanied by lower integrin activati

SUBMITTER: Heubel-Moenen FCJI 

PROVIDER: S-EPMC9303561 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature