Taming the massive genome of Scots pine with PiSy50k, a new genotyping array for conifer research.

ABSTRACT: Pinus sylvestris (Scots pine) is the most widespread coniferous tree in the boreal forests of Eurasia, with major economic and ecological importance. However, its large and repetitive genome presents a challenge for conducting genome-wide analyses such as association studies, genetic mapping and genomic selection. We present a new 50K single-nucleotide polymorphism (SNP) genotyping array for Scots pine research, breeding and other applications. To select the SNP set, we first genotyped 480 Scots pine samples on a 407 540 SNP screening array and identified 47 712 high-quality SNPs for the final array (called 'PiSy50k'). Here, we provide details of the design and testing, as well as allele frequency estimates from the discovery panel, functional annotation, tissue-specific expression pattern

SUBMITTER: Kastally C 

PROVIDER: S-EPMC9303803 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature