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Protocol to establish a stable MLL-AF9_AML mouse model.

ABSTRACT: Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse MLL-AF9_AML model to investigate AML. We have optimized the protocols for retrovirus infection, bone marrow transplantation (BMT), and leukemia monitoring to create a stable mouse model. In particular, we have used two rounds of BMT to enhance stability and efficiency. This model can be used to conduct drug administration and/or other interventions easily. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2022).

SUBMITTER: Lu J

PROVIDER: S-EPMC9304668 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Protocol to establish a stable <i>MLL-AF9</i>_AML mouse model.

Lu Jun J Zhao Huanhuan H Yang Lingling L Jiang Xi X

STAR protocols 20220718 3

Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse <i>MLL-AF9</i>_AML model to investigate AML. We have optimized the protocols for retrovirus infection, bone marrow transplantation (BMT), and leukemia monitoring to create a stable mouse model. In particular, we have used two rounds of BMT to enhance stability and efficiency. This model can be used to conduct drug administration and/or other interventions easily. For co ...[more]

PMID: 35874469

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