Transcriptional Integration of Distinct Microbial and Nutritional Signals by the Small Intestinal Epithelium.

ABSTRACT:

Background & aims

The intestine constantly interprets and adapts to complex combinations of dietary and microbial stimuli. However, the transcriptional strategies by which the intestinal epithelium integrates these coincident sources of information remain unresolved. We recently found that microbiota colonization suppresses epithelial activity of hepatocyte nuclear factor 4 nuclear receptor transcription factors, but their integrative regulation was unknown.

Methods

We compared adult mice reared germ-free or conventionalized with a microbiota either fed normally or after a single high-fat meal. Preparations of unsorted jejunal intestinal epithelial cells were queried using lipidomics and genome-wide assays for RNA sequencing and ChIP sequencing for the activating histone mar

SUBMITTER: Lickwar CR 

PROVIDER: S-EPMC9305020 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature