Human Cytomegalovirus-IE2 Affects Embryonic Liver Development and Survival in Transgenic Mouse.

ABSTRACT:

Background & aims

Congenital human cytomegalovirus (HCMV) infection is a common cause of liver injury. The major immediate-early protein 2 (IE2) of HCMV is critical for the progression of HCMV infection. As a result of species isolation, there are no animal models suitable for HCMV infection, which aimed to study the long-term effects of IE2 on embryonic liver development in vivo. Hence, this study aimed to investigate the role of IE2 in liver development using a transgenosis mouse model.

Methods

Rosa26-Loxp-STOP-Loxp (LAS)-IE2^+/-, cre mice that could specifically and stably express IE2 in the liver, were constructed. Phenotypic analysis, immunolocalization studies, messenger RNA analyses, transcriptome sequencing, and flow cytometry analysis were performed on Ros

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9305021 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature