Quantified integrated hepatitis B virus is related to viral activity in patients with chronic hepatitis B.

ABSTRACT:

Background and aims

HBV can integrate in the host genome of the hepatocyte and recent findings suggest that integrated HBV contributes to the persistent production of viral proteins. Here, we quantified the levels of integrated HBV in patients with chronic hepatitis B (CHB) and analyzed the relation between HBV integration, virological activity (plasma HBV DNA and HBsAg levels), and clinical outcomes.

Approach and results

We developed and validated a multistep Arthrobacter luteus (Alu)-PCR that specifically amplifies integrated HBV and RT-Alu-PCR detecting mRNA transcripts derived from integrated HBV. Pretreatment liver biopsy samples and baseline characteristics of 124 patients with CHB either treated for 48 weeks with pegylated interferon plus adefovir or tenofovir or rece

SUBMITTER: Erken R 

PROVIDER: S-EPMC9305117 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature