Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling.

ABSTRACT: Many life-science techniques and assays rely on selective labeling of biological target structures with commercial fluorophores that have specific yet invariant properties. Consequently, a fluorophore (or dye) is only useful for a limited range of applications, e.g., as a label for cellular compartments, super-resolution imaging, DNA sequencing or for a specific biomedical assay. Modifications of fluorophores with the goal to alter their bioconjugation chemistry, photophysical or functional properties typically require complex synthesis schemes. We here introduce a general strategy that allows to customize these properties during biolabelling with the goal to introduce the fluorophore in the last step of biolabelling. For this, we present the design and synthesis of 'linker' compounds, tha

SUBMITTER: Zhang L 

PROVIDER: S-EPMC9305292 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature