Development of dual reporter vector system for estimating translational activity of regulatory elements.

ABSTRACT:

Background

For the needs of modern biotechnology, a quantitative approach to the control of regulatory elements at all stages of gene expression has long become indispensable. Such a control regime is impossible without a quantitative analysis of the role of each regulatory element or pattern used. Therefore, it seems important to modify and develop the accuracy, reproducibility, and availability of methods for quantifying the contribution of each regulatory code to the implementation of genetic information.

Results

A new vector system for transient expression in plants is described; this system is intended for quantitative analysis of the contribution of regulatory elements to transcription and translation efficiencies. The proposed vector comprises two expression cassettes

SUBMITTER: Suhorukova AV 

PROVIDER: S-EPMC9306140 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature