Establishment of a piglet model for peritoneal metastasis of ovarian cancer.

ABSTRACT:

Background

A piglet model for peritoneal metastasis (PM) of ovarian cancer was developed. It will contribute to establishing innovative chemotherapeutical and surgical strategies without any limitation on rodent models.

Methods

A total of 12 four- to five-week-old piglets of 7 to 8 kg were used. Two phases of ovarian cancer cell injections were performed with laparoscopic surgery. In phase I trial, 5.0 × 10⁶ SK-OV-3 cells in 0.1 ml suspension were inoculated into the omentum, peritoneum, and uterine horns of two piglets twice with a one-week interval. In the phase II trial, 5.0 × 10⁶ SNU-008 cells in 0.1 ml suspension were injected only into uterine horns within the same time frame because tumor implantation after inoculation of SK-OV-3 cells was not ob

SUBMITTER: Lee EJ 

PROVIDER: S-EPMC9306172 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature