Genomic characterization of high-risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary-care hospital in Pakistan.

ABSTRACT:

Aims

Spread of carbapenem-resistant Enterobacterales have become a global problem. We characterized extended-spectrum β-lactamase (ESBL)-producing Enterobacterales from urinary tract infections cases from Allied Hospital Faisalabad, Pakistan.

Methods and results

Eleven (22%, 11/50) ESBL-producing Enterobacterales (Escherichia coli; n = 10 and Enterobacter hormaechei; n = 1) were recovered and processed through VITEK-2, PCR, rep-PCR followed by whole-genome sequencing (WGS) of ESBL-producing Ent. hormaechei and carbapenem-resistant E. coli isolates. Plasmid transferability of bla_NDM-1 -producers was assayed by conjugation experiments. All ESBL strains carried the bla_CTX-M-15 gene. Of these bla_CTX-M-15 producing E. coli, four also carried bla

SUBMITTER: Mohsin M 

PROVIDER: S-EPMC9306472 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature