Sphingosine-1-phosphate/TGF-β axis drives epithelial mesenchymal transition in asthma-like disease.

ABSTRACT:

Background and purpose

Airway remodelling is a critical feature of chronic lung diseases. Epithelial-mesenchymal transition (EMT) represents an important source of myofibroblasts, contributing to airway remodelling. Here, we investigated the sphingosine-1-phosphate (S1P) role in EMT and its involvement in asthma-related airway dysfunction.

Experimental approach

A549 cells were used to assess the S1P effect on EMT and its interaction with TGF-β signalling. To assess the S1P role in vivo and its impact on lung function, two experimental models of asthma were used by exposing BALB/c mice to subcutaneous administration of either S1P or ovalbumin (OVA).

Key results

Following incubation with TGF-β or S1P, A549 acquire a fibroblast-like morphology associated with an increase

SUBMITTER: Riemma MA 

PROVIDER: S-EPMC9306821 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature