Assessment of machine perfusion conditions for the donation after circulatory death heart preservation.

ABSTRACT:

Background

Donation after circulatory death (DCD) hearts requires machine perfusion preservation, the conditions of which are not well defined.

Methods

To achieve this, rat hearts were procured following a DCD or control beating-heart donation (CBD) model, and perfused for 60 min with one of three machine perfusion solutions-St. Thomas (ST), University of Wisconsin (UW), or Polyethylene Glycol-20k (PEG)-at one of two temperatures, 4°C or 15°C. At 15-min intervals, perfusion pressure was measured as a marker of vascular resistance. Colored microspheres were added to capture the distribution of perfusate into the metabolically active sub-endocardium, and the eluate was collected for troponin assays. Analyses compared groups using Wilcoxon rank-sum and ANOVA.

Results

Per

SUBMITTER: Cholyway R 

PROVIDER: S-EPMC9307000 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature