Dataset Information

Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.

ABSTRACT: Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated. We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling. We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P. We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated - a result with significant implications for the modelling of plant-soil-AMF interactions.

SUBMITTER: Keyes S

PROVIDER: S-EPMC9307049 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.

Keyes Sam S van Veelen Arjen A McKay Fletcher Dan D Scotson Callum C Koebernick Nico N Petroselli Chiara C Williams Katherine K Ruiz Siul S Cooper Laura L Mayon Robbie R Duncan Simon S Dumont Marc M Jakobsen Iver I Oldroyd Giles G Tkacz Andrzej A Poole Philip P Mosselmans Fred F Borca Camelia C Huthwelker Thomas T Jones David L DL Roose Tiina T Roose Tiina T

The New phytologist 20220215 2

Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated. We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in ...[more]

PMID: 35043984

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Project description:Interactions between plants and arbuscular mycorrhizal fungi (AMF) are strongly affected by soil phosphorus (P) availability. However, how P forms impact rhizosphere AMF diversity, community composition, and the co-occurrence network associated with native and invasive plants, and whether these changes in turn influence the invasiveness of alien species remain unclear. In this work, we performed a greenhouse experiment with the invasive species Solidago canadensis and its native congener S. decurrens to investigate how different forms of P altered the AMF community and evaluate how these changes were linked with the growth advantage of S. canadensis relative to S. decurrens. Plants were subjected to five different P treatments: no P addition (control), simple inorganic P (sodium dihydrogen phosphate, NaP), complex inorganic P (hydroxyapatite, CaP), simple organic P (adenosine monophosphate, AMP) and complex organic P (myo-inositol hexakisphosphate, PA). Overall, invasive S. canadensis grew larger than native S. decurrens across all P treatments, and this growth advantage was strengthened when these species were grown in CaP and AMP treatments. The two Solidago species harbored divergent AMF communities, and soil P treatments significantly shifted AMF community composition. In particular, the differences in AMF diversity, community composition, topological features and keystone taxa of the co-occurrence networks between S. canadensis and S. decurrens were amplified when the dominant form of soil P was altered. Despite significant correlations between AMF alpha diversity, community structure, co-occurrence network composition and plant performance, we found that alpha diversity and keystone taxa of the AMF co-occurrence networks were the primary factors influencing plant growth and the growth advantage of invasive S. canadensis between soil P treatments. These results suggest that AMF could confer invasive plants with greater advantages over native congeners, depending on the forms of P in the soil, and emphasize the important roles of multiple AMF traits in plant invasion.

Dataset Information

Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.

Publications

Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.

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