Multiplex PCR assay to detect high risk lineages of Salmonella Typhi and Paratyphi A.

ABSTRACT: Enteric fever infections remain a significant public health issue, with up to 20 million infections per year. Increasing rates of antibiotic resistant strains have rendered many first-line antibiotics potentially ineffective. Genotype 4.3.1 (H58) is the main circulating lineage of S. Typhi in many South Asian countries and is associated with high levels of antibiotic resistance. The emergence and spread of extensively drug resistant (XDR) typhoid strains has increased the need for a rapid molecular test to identify and track these high-risk lineages for surveillance and vaccine prioritisation. Current methods require samples to be cultured for several days, followed by DNA extraction and sequencing to determine the specific lineage. We designed and evaluated the performance of a new multip

SUBMITTER: Khokhar F 

PROVIDER: S-EPMC9307194 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature