Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering.

ABSTRACT: Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics involves introducing biomolecular cargoes into cells. However, successful delivery is a function of multiple experimental factors requiring several rounds of optimization. Here, we present a high-throughput multiwell-format localized electroporation device (LEPD) assisted by deep learning image analysis that enables quick optimization of experimental factors for efficient delivery. We showcase the versatility of the LEPD platform by successfully delivering biomolecules into different types of adherent and suspension cells. We also demonstrate multicargo delivery with tight dosage distribution and precise ratiometric control. Furthermore, we used the platform to achieve functional gene knockdown in h

SUBMITTER: Patino CA 

PROVIDER: S-EPMC9307252 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature