Accuracy of QuantiFERON SARS-CoV-2 research use only assay and characterization of the CD4⁺ and CD8⁺ T cell-SARS-CoV-2 response: comparison with a homemade interferon-γ release assay.

ABSTRACT:

Objectives

In this study, we aimed to characterize the SARS-CoV-2-specific T cell response detected by the QuantiFERON SARS-CoV-2 research use only assay in terms of accuracy and T cell subsets involved compared with a homemade interferon (IFN)-γ release assay (IGRA).

Methods

We evaluated T cell response by the standardized QuantiFERON SARS-CoV-2 tubes (antigen [Ag]1 and Ag2) and a homemade IGRA quantifying IFN-γ response to SARS-CoV-2 spike peptides (homemade-IGRA-SPIKE test). We evaluated the T cell subsets mediating the specific response using flow cytometry.

Results

We prospectively enrolled 66 individuals: COVID-19 or post-COVID-19 subjects and NO-COVID-19-vaccinated subjects, including healthy donors and immunocompromised subjects. The standardized kit detected

SUBMITTER: Aiello A 

PROVIDER: S-EPMC9307287 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature