Ontology highlight
ABSTRACT:
SUBMITTER: Chhipi-Shrestha JK
PROVIDER: S-EPMC9307672 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Chhipi-Shrestha Jagat K JK Yoshida Minoru M Iwasaki Shintaro S
STAR protocols 20220719 3
The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method. This protocol also details an alternative approach to monitor specific protein aggregates trapped in the filter membrane, by subsequent immunoblotting of ectopically expressed and endogenous proteins. For comp ...[more]