Vanadium pentoxide induced oxidative stress and cellular senescence in human lung fibroblasts.

ABSTRACT: Both environmental exposure to vanadium pentoxide (V₂O₅, V⁺⁵ for its ionic counterparts) and fibroblast senescence are associated with pulmonary fibrosis, but whether V⁺⁵ causes fibroblast senescence remains unknown. We found in a dose-response study that 2-40 μM V⁺⁵ caused human lung fibroblasts (HLF) senescence with increased senescence-associated β-galactosidase activity and p16 expression, while cell death occurred at higher concentration (LC₅₀, 82 μM V⁺⁵). Notably, measures of reactive oxygen species (ROS) production with fluorescence probes showed no association of ROS with V⁺⁵-dependent senescence. Preloading catalase (polyethylene-conjugated), a H₂O₂ scavenger, did not alle

SUBMITTER: He X 

PROVIDER: S-EPMC9307685 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature