Polymorphism rs11200638 enhanced HtrA1 responsiveness and expression are associated with age-related macular degeneration.

ABSTRACT:

Objectives

To investigate the role of polymorphism rs11200638 of high-temperature requirement factor A-1 (HtrA1) gene in the pathogenesis of age-related macular degeneration (AMD).

Methods

Cultured adult retinal pigment epithelial cells (ARPE-19) expressing HtrA1 gene were treated with H₂O₂ or lipopolysaccharides (LPS) and analysed using western blot and quantitative polymerase chain reaction to illustrate the effects of oxidative and inflammatory stress on HtrA1 gene expression. Luciferase reporter plasmid driven by HtrA1 promoter with either normal allele G or risk allele A at SNP rs11200638 was transfected to ARPE-19 cells to investigate the effect of the G/A variation on HtrA1 promoter activity. The effects of HtrA1 overexpression on ARPE-19 cells w

SUBMITTER: He F 

PROVIDER: S-EPMC9307815 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature