Spectral fiber photometry derives hemoglobin concentration changes for accurate measurement of fluorescent sensor activity.

ABSTRACT: Fiber photometry is an emerging technique for recording fluorescent sensor activity in the brain. However, significant hemoglobin absorption artifacts in fiber photometry data may be misinterpreted as sensor activity changes. Because hemoglobin exists widely in the brain, and its concentration varies temporally, such artifacts could impede the accuracy of photometry recordings. Here we present use of spectral photometry and computational methods to quantify photon absorption effects by using activity-independent fluorescence signals, which can be used to derive oxy- and deoxy-hemoglobin concentration changes. Although these changes are often temporally delayed compared with the fast-responding fluorescence spikes, we found that erroneous interpretation may occur when examining pharmacology

SUBMITTER: Zhang WT 

PROVIDER: S-EPMC9308135 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature