Construction and analysis of a Noccaea caerulescens TILLING population.

ABSTRACT:

Background

Metals such as Zn or Cd are toxic to plant and humans when they are exposed in high quantities through contaminated soil or food. Noccaea caerulescens, an extraordinary Zn/Cd/Ni hyperaccumulating species, is used as a model plant for metal hyperaccumulation and phytoremediation studies. Current reverse genetic techniques to generate mutants based on transgenesis is cumbersome due to the low transformation efficiency of this species. We aimed to establish a mutant library for functional genomics by a non-transgenic approach, to identify mutants with an altered mineral profiling, and to screen for mutations in bZIP19, a regulator of Zn homeostasis in N. caerulescens.

Results

To generate the N. caerulescens mutant library, 3000 and 5000 seeds from two sister plants o

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9308233 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature